c-6 disulfide  (Thermo Fisher)

Journal: Cell chemical biology

Article Title: Aptamers as reversible sorting ligands for preparation of cells in their native state

doi: 10.1016/j.chembiol.2019.12.004

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The sequences of all oligonucleotides generated in this study are listed in the and Table S3 and may therefore be synthesized, transcribed, or purchased from oligonucleotide vendors. table ft1 table-wrap mode="anchored" t5 caption a7 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-EGFR Antibody 1F4 Cell Signaling Technologies Cat# 2239 Rabbit monoclonal anti-EGFR Antibody D1D4J (PE Conjugate) Cell Signaling Technologies Cat# 48685 Rat monoclonal anti-EGFR Antibody ICR10 (FITC Conjugate) Abcam Cat# ab11400 Chemicals, Peptides, and Recombinant Proteins Dulbecco’s Phosphate Buffered Saline (DPBS) with MgCl 2 and CaCl 2 Sigma Cat# D8662–500ML Dulbecco’s Phosphate Buffered Saline (DPBS) without MgCl 2 or CaCl 2 Gibco Cat# 14190–144 Fetal Bovine Serum (not heat-inactivated) Gibco Cat# 16000–044 Heat-Inactivated Fetal Bovine Serum Gibco Cat# 10082–147 High Glucose Dulbecco’s Modified Eagle Medium Sigma Cat# D6429–500ML RPMI 1640 Gibco Cat# A10491 Salmon Testes DNA, Sodium Salt Millipore Sigma Cat# 262012–1GM Human EGFR protein extracellular domain G&P Biosciences Cat# FCL0169 Gibco EGF Recombinant Human Protein ThermoFisher Cat# PHG0315 eBioscience 10x RBC Lysis Buffer ThermoFisher Cat# 00-4300-54 5’-Biotin-G-Monophosphate TriLink Biotechnologies Cat# N-6003 5’-Thiol-C-6 Disulfide Modifier CED phosphoramidite ChemGenes Cat# CLP-8506 Biotin (BB) CED phosphoramidite ChemGenes Cat# CLP-1517 5’-DMT-2’-TOM-ribo Adenosine (n-acetyl) OP ChemGenes Cat# ANP-3201 5’-DMT-2’-TOM-ribo Guanosine (n-acetyl) OP ChemGenes Cat# ANP-3203 2’-Fluoro-2’-deoxy Cytidine (n-ac) CED phosphoramidite ChemGenes Cat# ANP-9152 2’-Fluoro-2’-deoxy Uridine CED phosphoramidite ChemGenes Cat# ANP-9154 Triethylamine Acetate (TEAA) 2.0M, HPLC grade Glen Research Cat# 60-4110-52 Acetonitrile for HPLC, gradient grade, ≥99.9% Sigma Aldrich Cat# 34851–4×4L Critical Commercial Assays Streptavidin, Alexa Fluor™ 647 Conjugate ThermoFisher Scientific Cat# S21374 Streptavidin, Alexa Fluor™ 488 Conjugate ThermoFisher Scientific Cat# S32354 Vybrant™ DiI cell-labeling solution ThermoFisher Scientific Cat# V-22885 LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit ThermoFisher Scientific Cat# {"type":"entrez-nucleotide","attrs":{"text":"L34975","term_id":"522218"}} L34975 Experimental Models: Cell Lines A431 cells Duke Cell Culture Facility n/a Jurkat cells Duke Cell Culture Facility n/a Oligonucleotides Biotin E07 (RNA) 5’-Biotin linker-GGA fCGG AfUfU fUAA fUfCG fCfCG fUAG AAA GfCA fUGfU fCAA AGfC fCGG AAfC fCGfU fCfC idT-3’ Synthesized for this paper.

Techniques: Recombinant, Modification, Lysis, Staining, Cell Culture, Synthesized, Software